Saccharomyces genus as a model of evolution and industrial applications
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Saccharomyces genus offers a huge diversity that can be mined for different industrial applications (wine, beer, biofuels, and others). MITOGRESSION seeks to develop new strains via hybridization and different mitochondrial-nuclear combinations.
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Saccharomyces genus as a model of evolution and industrial applications
- 1. Saccharomyces genus as a model of evolution and industrial applications David Peris, Postdoctoral Marie Curie Fellow Biotechnology Department, SBYBI Group 8th February 2019 @djperis
- 2. Saccharomyces cerevisiae is the workhorse in the alcoholic industry Ales Traditional beverages Wine Cider Sake
- 3. Beyond Saccharomyces cerevisiae Scannell et al 2010 Libkind et al 2011 Liti et al 2013 Naseeb et al 2018 S. paradoxus S. mikatae S. arboricola S. kudriavzevii S. uvarum S. cerevisiae S. eubayanus S. jureii
- 4. SBYBI favorite strains Henriques et al 2018 Alonso del Real et al 2017 Pérez-Torrado et al 2016 Peris et al 2016 Oliveira et al 2014 Gamero et al 2013 Tronchoni et al 2012 Salvadó et al 2011 Tronchoni et al 2009 S. paradoxus S. mikatae S. arboricola S. kudriavzevii: CR85 S. uvarum: BMV58 S. cerevisiae: T73 S. eubayanus S. jureii
- 5. S. cer T73 Ethanol tolerant Good fermentative profile High temperature profile High ethanol production High glycerol production Low ethanol production Acceptable fermentative profile Low temperature profile Cellulolytic activity Production of secondary flavors Low ethanol tolerance S. kud CR85 S. uva BMV58 High glycerol production Low ethanol production Low acetic acid production Low temperature profile Higher fructose transport Production of secondary flavors Low fermentative efficiency SBYBI favorite strains: fermentative properties
- 6. General phenotypic trends using more strains Peris et al In preparation Optimumtemperature
- 7. S. cerevisiae S. paradoxus S. mikatae S. jurei S. kudriavzevii S. arboricola S. uvarum S. eubayanus Species Hybridization as a domestication mechanism Peris et al 2018 Pérez-Torrado et al 2015 Pérez-Través et al 2014 Peris et al 2012a,b,c Lopes et al 2010 Arroyo-Lopez et al 2010 Tronchoni et al 2009 Belloch et al 2008 Gonzalez et al 2008 Gonzalez et al 2007
- 8. One mechanism to generate hybrids Peris et al 2018
- 9. One mechanism to generate hybrids
- 10. Mitochondrial genome is quickly fixed
- 11. 94% of industrial hybrids inherited the non-cerevisiae genome Langdon, Peris et al In preparation Peris et al 2018 Peris et al 2016
- 12. ~120 Industrial Hybrids Mitochondrial inheritance might drive genomic final output %ofgenomeretained nuclear genome Langdon, Peris et al In preparation Peris et al 2018 Peris et al 2012b,c
- 13. Generating yeast diversity by mitochondrial introgression for wine innovation MITOGRESSION
- 14. Aims Does mitochondrial genome influence in the genome stabilization? What is the impact of mitochondrial inheritance in the phenotypes?
- 15. 6 species hybrids Peris et al In preparation
- 17. Mitochondrial inheritance impacts in the genome output in synthetic hybrids Mitochondria %ofS.cerevisiaegenome 100 80 60 40 Parent Sc Construction Evolved Peris et al In preparation
- 18. Mitochondrial inheritance directly related with temperature adaptation Baker, Peris et al 2019 Li, Peris et al 2019
- 19. 19 Generation of more hybrids
- 20. 20 Synthetic Must Sugar consumption Ethanol production Glycerol production Acetic acid production Oxidative tolerance Ethanol tolerance Temperature tolerance Respiration Osmotic tolerance Higher alcohols Esters Kinetics Tasting panel (aroma, taste/mouthfeel, overall impression) or/and HPLC GC Mass Loss Generation of more hybrids & Test of other conditions pH Aminoacid content Micronutrients (Fe, S)
- 21. Amparo Querol Lainy Ramírez Alejandro Aguilar Laura Pérez David Lázaro Seba Tapia Carla Perpiñá Querol Lab Members Eladio Barrio Barrio Lab Members Justin Fay Li Xueying Chris T. Hittinger’s lab Quinn Langdon EmilyClaire Baker Russell Wrobel Ryan Moriarty Hittinger Lab Members UW & GLBRC CollaborationSBYBI José Guillamón Guillamón Lab Members Maite Martínez Sergi Puig Raquel Sorribes Sergi Lab Members