Abstract
Quinacrine staining of somatic chromosomes in Allium carinatum shows intense fluorescence patterns which allow their recognition and the study of their degree of heterozygosity. This makes possible the study of chromosome polymorphism at a level until now impossible to achieve. The intense fluorescence patterns correspond to heterochromatic segments visible as darkly stained regions in prophase chromosomes. Interphase nuclei show fluorescent chromocentres of the same size and distribution as in conventionally stained preparations, and there is a good correlation between intense fluorescence patterns, late replicating DNA and heterochromatin.
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Caspersson, T., Zech, L., Modest, E.J., Foley, G.E., Wagh, U., Simonsson, E.: Chemical differentiation with fluorescent alkylating agents in Vicia faba metaphase chromosomes. Exp. Cell Res. 58, 128–140 (1969).
Darlington, C.D., La Cour, L.F.: The handling of chromosomes, 5th ed. London: Allen an.d Unwin 1969.
Lerman, LS.: Structural considerations in the interaction of DNA and acridines. J. molec. Biol 3, 18–30 (1961).
—: The structure of the DNA-acridine complex. Proc. nat. Acad. Sci. (Wash.) 49, 94–102 (1963).
Nagl, W.: Der mitotische und endomitotische Kernzyklus bei Allium carinatum. I. Struktur, Volumen und DNS-Gehalt der Kerne. Öst. bot. Z. 115, 322–353 (1968).
—: The mitotic and endomitotic nuclear cycle in Allium carinatum II. Relations between DNA replication and chromatin structure. Caryologia (Firenze) 23, 71–78 (1970).
Neville, D.M., Davies, D.R.: The interaction of acridine dyes with DNA. An X-ray diffraction and optical investigation. J. molec. Biol. 17, 57–74 (1966).
Pearson, P.L., Bobrow, M., Vosa, C.G., Barlow, P.W.: Quinacrine fluorescence in mammalian chromosomes. Nature (Lond.) (in press 1971).
Tubbs, R.K., Ditmars, W.E., Winkle, Q. van: Heterogeneity of the interaction of DNA with acriflavine. J. molec. Biol. 9, 545–557 (1964).
Vosa, C.G.: Heterochromatin recognition with fluorochromes. Chromosoma (Berl.) 30, 366–372 (1970a).
—: The discriminating fluorescence patterns of the chromosomes of Drosophila melanogaster. Chromosoma (Berl.) 31, 446–451 (1970b).
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Vosa, C.G. The quinacrine-fluorescence patterns of the chromosomes of Allium carinatum . Chromosoma 33, 382–385 (1971). https://doi.org/10.1007/BF00326500
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DOI: https://doi.org/10.1007/BF00326500