A Study On Anthelmintic Activity of Canavalia Ensiformis (Jack Bean) Pods

Volume 5, Issue 10, October – 2020 International Journal of Innovative Science and Research Technology
ISSN No:-2456-2165
A study on Anthelmintic Activity of Canavalia

Ensiformis (Jack bean) Pods
Ankita Kotkar, Student (M. pharm) Quality Assurance.
Dr. C. J. Bhangale principal PRES'S College of Pharmacy Chincholi, Nashik.
Vandana P. Aher. dept-Pharmacognosy PRES'S college of Pharmacy Chincholi Nashik.
Kaveri Vaditke. Dept. Chemistry PRES'S college of Pharmacy Chincholi, Nashik.
Student Department of M.pharmacy, Savitribai Phule University, Pune, India.
PRES'S College of Pharmacy Chincholi, Nashik, Maharashtra, India.
Abstract:- Various species of canavalia Ensiformis have Anthelmintic activity against Earthworms and Flatworms.
been extensively studied for their Nutritional evaluation. The activities of extract we're compared with standard drug
Canavalia Ensiformis is expected to possess many Piperazine Citrate.
constituents among them tannins, saponins are major
constitutes.so due to presence of tannins Canavalia II. EXPERIMENTAL WORK
Ensiformis may possess Anthelmintic activity. In present
work Metabolic and Aqueous extract of pods of  Material and Method:
canavalia Ensiformis were evaluated for its Anthelmintic
activity against earthworm at four different
 Collection And Identifications of Pods:
concentration1, 2, 5, 10 mg/ml. The Anthelmintic activity
Pods of canavalia Ensiformis were collected from
of aqueous extract and Methanolic extract was
local grocery market Nashik, Maharashtra, India. The
comparable with standard drug (Piperazine Citrate
identification of plant was done at KTHM College Nashik,
1mg/ml).Worms are collected and washed with normal
Maharashtra.
saline solution and kept in phosphate buffered saline
solution pH 7.5 to 8.0 until further use. Standard drug
solution was prepared in Distilled water. Activity was  Pretreatment of Pods:
evaluated by the time required for paralysis and death of The pods was initially washed with simply water then
worms by extracts. The data shows Methanolic extract dried in sunlight under shade for 20-25 days until pods
possess comparable Anthelmintic activity with standard becomes completely dry. The dried pods were taken for
drug. The result shows that the plant has the potential to further extraction procedure.
be used as Anthelmintic.
 Preparation of Aqueous Extract:
Keywords:- Canavalia Ensiformis, Anthelmintic Activity, Accurately weighed 100 gm powder taken in stainless
Earthworms, Flatworms. steel vessel and mixed with 500ml distilled water the
mixture was boiled for about 3 hours using gas burner.
Filtration of mixture was carried out. Filtrate was taken into
I. INTRODUCTION
a beaker and evaporated until it converted into semisolid
concentrate liquor. After that it was dried completely to get
Canavalia Ensiformis commonly known as (Jack bean)
dried residue of the extract we're collected by using same
or in Marathi known as Abai. Which is not official in
method describe in preparation of Methanolic extract.
Ayurvedic pharmacopoeia.it is one of the excellent plant for
human being made and gifted by the nature having
composition of all the essential constituents that are required  Preparation of Methanolic Extract:
for normal and good human health. The flavonoids we're Take 60 gm of powder of drug was packed in thimble
found to have antimicrobial activity. The phytochemical flask and 550 ml of Methanol was added in 1 liter round
investigation on Canavalia Ensiformis have revealed the bottom flask the soxhlet assembly was set up the extract was
presence of tannins, saponins, flavonoids, cardiac glycosides. filtered and filtrate was concentrate up to 50ml using water-
It is also good source of mineral and acid. The seeds of bath. Filtration of mixture was carried out. Filtrate was taken
canavalia Ensiformis contain canavalin A. Canavalia ensure into a beaker and evaporated until it converted into
also used to treat vomiting ,obesity, stomach ache and semisolid concentration liquor after that it was dried
inflammatory disease but proper scientific studies have not completely to get dried residue, which can be used to
been much reported for this plant especially for an. prepare the stock solution (w/v) and the %yield would be
Anthelmintic perspective. In present work Methanolic and 12.2%w/w and stored in the freezer until further use.
aqueous extract of canavalia Ensiformis pods evaluated for
IJISRT20OCT400 www.ijisrt.com 798

ISSN No:-2456-2165
III. SCREENING OF ANTHELMINTIC ACTIVITY: IV. RESULT AND DISCUSSION:
The Anthelmintic activity of pods extract of canavalia The result in table 1 shows that the time taken for
Ensiformis has been evaluated by on adult Indian paralysis and death of worms after treating with the test
Earthworm.it was collected during rainy season. Worms are substance. The activity was comparable with standard drug
collected and washed with normal saline solution and kept Piperazine citrate. Methanolic and aqueous extract both
in phosphate buffered saline solution pH 7.5 to 8.0 until shows significant Anthelmintic activity against Earthworms.
further use. All the working solution are freshly prepared Methanolic extract found to be more active as compared to
before start of experiment. Take actively moving water extract the ME extract demonstrated paralysis as well
earthworms placed k Petri dishes at room temperature 25°C as death of worms in comparative good time as compared to
to 30°C. Containing 1mg/ml,2mg/ml,5mg/ml,10mg/ml of Piperazine Citrate especially at high concentration of
Aqueous and Methanolic extract in a phosphate buffer saline 10mg/ml in case of canavalia Ensiformis. While water
solution positive and negative controls with Piperazine extract shows significant activity phytochemical analysis of
citrate. Three replicate should be done for each the crude extract revealed presence of Tannins, Saponins,
concentration o and observation made at 1,2,3,4,5,6,7 and and flavonoids. The tannins are known to have Anthelmintic
12 hours of time to get paralyzed and finally die for all the activity.
worms. After each of the experiment groups the respective
extract concentration solution was discarded and number of V. CONCLUSION
alive and dead worms in each concentration was counted the
worms counted as dead when they lost their motility In conclusion the use of pods of canavalia Ensiformis
permanently and did not recover even after placing in as Anthelmintic have been confirmed as the pods extract
phosphate buffer saline solution. Paralysis was followed by displayed activity against the worms in the study.
fading away of the body color of the dead worms.

ISSN No:-2456-2165

ISSN No:-2456-2165
Treatment Conc.mg/ml Paralysis time(min) Death time (min)

(Mean ± SEM) (Mean ± SEM)
Control _ 0.0 0.0
Piperazine Citrate 1 32±2.3 57.3±5.5
1 140.0±1.1 178.7±1.9
Aqueous Extract 2 159.7±2.0 198.0±2
5 83.3±0.9 119.0±2.6
10 109.3±1.8 154.3±1.2
1 120.0±1.1 160.7±1.8
Methanolic Extract 2 139.0±0.6 175.3±2.0
5 66.0±1.5 96.0±2.1
10 100.3±0.9 147.7±3.5
Table1

ISSN No:-2456-2165
REFERENCES
[1]. Nutritional evaluation of canavalia Ensiformis

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[2]. Physicochemical characterization of Jack bean
(canavalia Ensiformis): David Betancur_Ancona,
Gwendoline perazamercodo Yolanda moquel-ordonez:
food chemistry 84(2), 287-295, and 2004.
[3]. Three dimensional structure of canavalin A from Jack
bean: Tzu-ping KO, Joseph Dang, Alexander Mc
person: Plant physiology 101(3), 729-744, 1993.
[4]. Some physicochemical and antinutrutional properties
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[5]. Species/variety difference in biochemical composition
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[9]. Scholar’ s academic journals of pharmacy.

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Volume 5, Issue 10, October – 2020 International Journal of Innovative Science and Research Technology

A study on Anthelmintic Activity of Canavalia

IJISRT20OCT400 www.ijisrt.com 798

IJISRT20OCT400 www.ijisrt.com 799

IJISRT20OCT400 www.ijisrt.com 800

Treatment Conc.mg/ml Paralysis time(min) Death time (min)

IJISRT20OCT400 www.ijisrt.com 801

[1]. Nutritional evaluation of canavalia Ensiformis

IJISRT20OCT400 www.ijisrt.com 802

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