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Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.)

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Abstract

A complete method to regenerate adventitious shoots and to produce field-ready trees from three commercial cultivars of sweet cherry (Prunus avium L.) is described. The effects of explant types, pre-treatments, basal media, and phloroglucinol on cultivars Bing, Sweetheart, and Lapins were investigated. Callus developed on four explant types: apical shoot tips isolated from orchard trees; and punctured shoot tips, stem sections, and shoot bases of in vitro shoot cultures. Callus formed on Bing (5%), Sweetheart (8%), and Lapins (20%) shoot tips from orchard trees after 4 months on Murashige and Skoog medium (MS) at half-strength with 3 μM benzylaminopurine (BA). In vitro-derived explants formed callus after 3 months on Woody Plant Medium with 3 μM BA (W3B): punctured shoot tips (Sweetheart and Lapins 67%), stem sections (Sweetheart 31%, Lapins 27%), and shoot bases (Sweetheart 10%, Lapins 17%). Pre-treatment of shoot cultures on MS with 3 μM BA and 1 mM phloroglucinol increased callus formation three-fold on shoot base explants. Callus was separated from parental explants and maintained on MS with 3 μM BA. Shooting was induced by transferring callus to W3B. At 2 weeks, shoot development approached 100%. By 4 weeks, 7–17 shoots had formed on each explant. Callus was maintained for 1.5 years with no decrease in shoot production. Shoots were grafted onto Mazzard (P. avium) rootstocks with 54% (Sweetheart), 57% (Lapins), and 21% (Bing) success after 5 weeks.

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Abbreviations

BA:

6-benzylaminopurine

2,4-D:

2,4-dichlorophenoxyacetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog medium

NAA:

α-naphthaleneacetic acid

WPM:

Woody Plant Medium

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Acknowledgements

This project was funded by the Industrial Research Assistance Program and was carried out in collaboration with Agriculture and Agri-Food Canada. Certified virus-free shoots were donated by the Okanagan Plant Improvement Company. We thank S. Wahlgren for valuable advice, M. Weiss for photography, and E. Randall and D. Weir.

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Authors and Affiliations

  1. Okanagan Specialty Fruits Inc., PO Box 1533, Summerland, British Columbia, Canada, V0H 1Z0

    Mistianne Feeney

  2. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada, V0H 1Z0

    Basdeo Bhagwat & W. David Lane

  3. Department of Behavioural Ecology, Institute of Zoology, University of Bern, 3032, Bern, Switzerland

    Jeremy S. Mitchell

Authors
  1. Mistianne Feeney
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  2. Basdeo Bhagwat
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  3. Jeremy S. Mitchell
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  4. W. David Lane
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Correspondence to Mistianne Feeney.

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Feeney, M., Bhagwat, B., Mitchell, J.S. et al. Shoot regeneration from organogenic callus of sweet cherry (Prunus avium L.). Plant Cell Tiss Organ Cult 90, 201–214 (2007). https://doi.org/10.1007/s11240-007-9252-1

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  • DOI: https://doi.org/10.1007/s11240-007-9252-1

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