Cheltenham Town were beaten by an Accrington Stanley goal scored after 30 seconds as they suffered their second away defeat of the season.
Sean McConville touched over the line from close range after Shaun Whalley raced through on goal and it was enough to seal Stanley's first home win of the campaign.
Cheltenham did stage a late push for a leveller, but they could not find a way through, with Alfie May used from the bench for the second game in a row.
It was the Robins' first league defeat at Accrington for 13 years.
Cheltenham play Walsall at home in the Papa John's Trophy group stage on Tuesday night.
Accrington Stanley (4-1-4-1): Jensen; Clark, Astley, Tharme, Rodgers (Sloane 59); Coyle; McConville, Conneely, Pritchard, Whalley; Leigh (Hamilton 75). Subs not used: Savin, Sangare, Nolan, Martin, Fernandes.
Cheltenham Town (3-5-2): Southwood; Long, Taylor, Freestone; Jackson (Norton 67), Sercombe, Perry, Broom, Ferry (Williams 67); Lloyd (May 57), Nlundulu. Subs not used: MacDonald, Raglan, Barkers, Chapman.
Referee: Stephen Martin.
Attendance: 1,996 (137 away).
See how the action unfolded in our live blog below
Final seconds
Here, Accrington look like they have won at home for the first time this season
Accrington break
After some Cheltenham pressure and Freestone does well to block a shot and it's a corner to Stanley
Five to play
Cheltenham have created very little, but set pieces/long throws have caused some issues for the home side
Yellow card
For Jackson after a foul on Whalley, 53rd minute. Accrington broke at pace after Sercombe's corner was cleared
Close
Broom's shot deflected just wide for a corner, after Jackson's low effort blocked, 52nd minute
McConville
Free header, Southwood saves well, 46th minute and again Cheltenham slow starting the half
